Journal: Reproduction in domestic animals = Zuchthygiene

Article Title: Localization of orexin B and receptor 2 for orexins in testicular cytotypes of the camelid alpaca (Vicugna pacos).

doi: 10.1111/rda.12931

Figure Lengend Snippet: FIGURE 3 OX2-IR in cytotypes of the alpaca testis. (a): Small cluster of Leydig cells containing different quantity of small positive granules scattered in their cytoplasm. (b, c): Round (b) and elongated (c) spermatids showed in their cytoplasm arciform or roundish positive structures which, respectively, embraced portion of the nucleus of the younger cells or were localized in the tail of the older ones. Avidin–biotin immunohistochemical technique. Bars: 20 μm

Article Snippet: Mouse anti- OxA (MAB763) and anti- OxB (MAB734) monoclonal antibodies and their synthetic peptides were obtained from R&D Systems (Abingdon, UK) and from Tocris Bioscience (Bristol, UK), respectively; goat polyclonal anti- OX2 antibody (sc- 8074) and its blocking peptide (sc- 8074 P) from Santa Cruz Biotechnologies (Santa Cruz, CA, USA); horseradish peroxidase- conjugated rabbit anti- goat IgG (A- 5420) and bovine serum albumin (BSA) from Sigma Chemical Co. (St. Louis, MO, USA); and biotinylated goat anti- mouse (BA- 9200), rabbit anti- goat (BA- 1000) secondary antibodies and avidin–biotin complex (PK- 6105) from Vector Laboratories (Burlingame, CA, USA).

Techniques: Avidin-Biotin Assay, Immunohistochemical staining

Journal: Reproduction in domestic animals = Zuchthygiene

Article Title: Localization of orexin B and receptor 2 for orexins in testicular cytotypes of the camelid alpaca (Vicugna pacos).

doi: 10.1111/rda.12931

Figure Lengend Snippet: FIGURE 4 OX2 expression detected by means of Western blotting analysis of testis homogenate. Lane 1: alpaca testis. Lane 2: rat brain. Molecular weight markers are expressed in kDa and reported on the right

Article Snippet: Mouse anti- OxA (MAB763) and anti- OxB (MAB734) monoclonal antibodies and their synthetic peptides were obtained from R&D Systems (Abingdon, UK) and from Tocris Bioscience (Bristol, UK), respectively; goat polyclonal anti- OX2 antibody (sc- 8074) and its blocking peptide (sc- 8074 P) from Santa Cruz Biotechnologies (Santa Cruz, CA, USA); horseradish peroxidase- conjugated rabbit anti- goat IgG (A- 5420) and bovine serum albumin (BSA) from Sigma Chemical Co. (St. Louis, MO, USA); and biotinylated goat anti- mouse (BA- 9200), rabbit anti- goat (BA- 1000) secondary antibodies and avidin–biotin complex (PK- 6105) from Vector Laboratories (Burlingame, CA, USA).

Techniques: Expressing, Western Blot, Molecular Weight

Journal: medRxiv

Article Title: multiSero: open multiplex-ELISA platform for analyzing antibody responses to SARS-CoV-2 infection

doi: 10.1101/2021.05.07.21249238

Figure Lengend Snippet: (A) Image of well containing Scienion-printed antigen array. Array layout shows the relative locations of SARS-CoV-2 antigens included in the array: receptor binding domain of spike-RBD (RBD; green), spike (ochre), and nucleoprotein (N; pink). Each antigen was spotted at two concentrations which are indicated in the right panel of the layout. Black spots represent anti-kappa-biotin fiducials, mauve spots represent anti-IgG Fc, and grey spots represent GFP foldon. (B) Pysero first detects center points of all spots in the cropped image (red x). Fiducial positions (blue +) are initialized based on detected spot coordinates. Coordinate transformation that registers the initial fiducials with detected fiducials is estimated using particle filtering. A grid containing all spot locations (green) in the antigen layout is then transformed onto the image using the estimated coordinate transformation. The coordinates of each spot are then used to crop individual spots and extract their OD. (C) Comparison of Scienion analysis and pysero spot detection. Original image of a well imaged by SciREADER CL2 includes comets (white arrow) and fiber-like debris. Center image is the output of Scienion spot detection and analysis. A black spot indicates an analyte-containing spot was not found at the location, green spots indicate positive spot value, and blue spots indicate the location of fiducial spots. The size of the spot indicates the area analyzed by Scienion. Right image is the output of pysero. Green dots indicate registered grid positions (size of marker unrelated to area analyzed), blue crosses indicate initialized fiducial spots.

Article Snippet: Sample was aspirated and wells incubated for 1 hour with 100 uL of an even mixture of biotinylated goat antibodies targeting the kappa (25 ng mL −1 , Southern Biotech cat. 2070-08) and lambda (25 ng mL −1 Southern Biotech cat. 2060-08) light chains.

Techniques: Binding Assay, Transformation Assay, Marker

Journal: Autoimmunity

Article Title: B cell TLR1/2, TLR4, TLR7 and TLR9 interact in induction of class switch DNA recombination: modulation by BCR and CD40, and relevance to T-independent antibody responses

doi: 10.3109/08916934.2014.993027

Figure Lengend Snippet: Antibodies.

Article Snippet: Applications Mouse B220, PE-conjugated Rat mAb eBioscience 25-0452-83 FCM Mouse B220, APC-conjugated Rat mAb BD Pharmingen 553092 FCM Mouse CD138, biotin-conjugated Rat mAb BD Pharmingen 553713 FCM Mouse Ig Goat pAb Southern Biotech 1012-01 ELISA Mouse IgG Goat pAb Southern Biotech 1030-01 ELISA Mouse IgM, FITC-conjugated Rat mAb BD Pharmingen 553437 FCM Mouse IgG1, FITC-conjugated Rat mAb BD Pharmingen 553443 FCM Mouse IgG1, APC-conjugated Rat mAb BD Pharmingen 550874 FCM Mouse IgG1, biotin-conjugated Goat pAb Southern Biotech 1070-08 ELISA Mouse IgG2a, FITC-conjugated Rat mAb BD Pharmingen 553390 FCM Mouse IgG2b, biotin-conjugated Goat pAb Southern Biotech 1090-08 FCM Mouse IgG3, FITC-conjugated Rat mAb BD Biosciences 553403 FCM Mouse IgG3, biotin-conjugated Goat pAb Southern Biotech 1100-08 ELISA Mouse IgA, FITC-conjugated Rat mAb BD Pharmingen 559354 FCM Mouse IgA, biotin-conjugated Goat pAb Southern Biotech 1040-08 FCM, ELISA Mouse IgE, PE-conjugated Rat mAb eBioscience Dec-92 FCM Open in a separate window Abbreviations: mAb, monoclonal antibody; pAb, polyclonal antibody; FCM, flow cytometry; ELISA, enzyme-linked immunosorbent assay.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Autoimmunity

Article Title: B cell TLR1/2, TLR4, TLR7 and TLR9 interact in induction of class switch DNA recombination: modulation by BCR and CD40, and relevance to T-independent antibody responses

doi: 10.3109/08916934.2014.993027

Figure Lengend Snippet: NP-LPS induces long-term and secondary antibody response in vivo. Three C57BL/6 mice (8-12 week old) were injected intraperitoneally with NP-LPS (25 μg) in PBS at d 0 and re-injected with NP-LPS (10 μg) in PBS at d 90 (second arrow). Sera were collected prior to the first injection and at different days, and analyzed for titers of NP3-binding IgG2b and IgG3 (depicted as relative units). Titers at d 0 were beyond the detection limit and set as 1 for the graph purpose.

Article Snippet: Applications Mouse B220, PE-conjugated Rat mAb eBioscience 25-0452-83 FCM Mouse B220, APC-conjugated Rat mAb BD Pharmingen 553092 FCM Mouse CD138, biotin-conjugated Rat mAb BD Pharmingen 553713 FCM Mouse Ig Goat pAb Southern Biotech 1012-01 ELISA Mouse IgG Goat pAb Southern Biotech 1030-01 ELISA Mouse IgM, FITC-conjugated Rat mAb BD Pharmingen 553437 FCM Mouse IgG1, FITC-conjugated Rat mAb BD Pharmingen 553443 FCM Mouse IgG1, APC-conjugated Rat mAb BD Pharmingen 550874 FCM Mouse IgG1, biotin-conjugated Goat pAb Southern Biotech 1070-08 ELISA Mouse IgG2a, FITC-conjugated Rat mAb BD Pharmingen 553390 FCM Mouse IgG2b, biotin-conjugated Goat pAb Southern Biotech 1090-08 FCM Mouse IgG3, FITC-conjugated Rat mAb BD Biosciences 553403 FCM Mouse IgG3, biotin-conjugated Goat pAb Southern Biotech 1100-08 ELISA Mouse IgA, FITC-conjugated Rat mAb BD Pharmingen 559354 FCM Mouse IgA, biotin-conjugated Goat pAb Southern Biotech 1040-08 FCM, ELISA Mouse IgE, PE-conjugated Rat mAb eBioscience Dec-92 FCM Open in a separate window Abbreviations: mAb, monoclonal antibody; pAb, polyclonal antibody; FCM, flow cytometry; ELISA, enzyme-linked immunosorbent assay.

Techniques: In Vivo, Injection, Binding Assay

Journal: Autoimmunity

Article Title: B cell TLR1/2, TLR4, TLR7 and TLR9 interact in induction of class switch DNA recombination: modulation by BCR and CD40, and relevance to T-independent antibody responses

doi: 10.3109/08916934.2014.993027

Figure Lengend Snippet: LPS induces B cells to undergo CSR and plasma cell differentiation in vitro in a dose- and time-dependent manner (flow cytometry analysis). (a, b) CSR to IgG1 in B cells stimulated with LPS at the indicated concentrations plus IL-4 for 4 d (a, top panels), and formation of B220loCD138hi plasma cells in the same cell culture (a, bottom panels). Mean and s.d. of data from four independent experiments were depicted in (b). p values calculated by paired student t test. (c) CSR to IgG1 in B cells stimulated with LPS plus IL-4, starting at d 0, and harvested at d 2, d 3, d 4, d 5 and d 7, as indicated. (d) Formation of B220loCD138hi plasma cells (top panels) as well as the proportion of IgG1+ (middle panels) and IgM+ (bottom panels) within those plasma cells in cultures of B cells stimulated with LPS plus IL-4, starting at d 0, and harvested at d 2, d 3, d 4, d 5 and d 7, as indicated. (e) CSR to IgG1 in B cells stimulated with LPS plus IL-4 for the period indicated, pelleted to remove LPS, and then cultured in RPMI-FBS only until being harvested at d 4. Data in (a, c, d, e) are representative of three independent experiments.

Article Snippet: Applications Mouse B220, PE-conjugated Rat mAb eBioscience 25-0452-83 FCM Mouse B220, APC-conjugated Rat mAb BD Pharmingen 553092 FCM Mouse CD138, biotin-conjugated Rat mAb BD Pharmingen 553713 FCM Mouse Ig Goat pAb Southern Biotech 1012-01 ELISA Mouse IgG Goat pAb Southern Biotech 1030-01 ELISA Mouse IgM, FITC-conjugated Rat mAb BD Pharmingen 553437 FCM Mouse IgG1, FITC-conjugated Rat mAb BD Pharmingen 553443 FCM Mouse IgG1, APC-conjugated Rat mAb BD Pharmingen 550874 FCM Mouse IgG1, biotin-conjugated Goat pAb Southern Biotech 1070-08 ELISA Mouse IgG2a, FITC-conjugated Rat mAb BD Pharmingen 553390 FCM Mouse IgG2b, biotin-conjugated Goat pAb Southern Biotech 1090-08 FCM Mouse IgG3, FITC-conjugated Rat mAb BD Biosciences 553403 FCM Mouse IgG3, biotin-conjugated Goat pAb Southern Biotech 1100-08 ELISA Mouse IgA, FITC-conjugated Rat mAb BD Pharmingen 559354 FCM Mouse IgA, biotin-conjugated Goat pAb Southern Biotech 1040-08 FCM, ELISA Mouse IgE, PE-conjugated Rat mAb eBioscience Dec-92 FCM Open in a separate window Abbreviations: mAb, monoclonal antibody; pAb, polyclonal antibody; FCM, flow cytometry; ELISA, enzyme-linked immunosorbent assay.

Techniques: Cell Differentiation, In Vitro, Flow Cytometry, Cell Culture

Journal: Autoimmunity

Article Title: B cell TLR1/2, TLR4, TLR7 and TLR9 interact in induction of class switch DNA recombination: modulation by BCR and CD40, and relevance to T-independent antibody responses

doi: 10.3109/08916934.2014.993027

Figure Lengend Snippet: LPS-induced CSR to IgG1 is enhanced by Pam3CSK4 and CpG in the presence of BCR signaling (flow cytometry analysis). (a) CSR to IgG1 in CFSE-labeled B cells stimulated with suboptimal doses of LPS, Pam3CSK4, R-848 and CpG (as indicated), alone or in pair-wise combination, all in the presence of anti–δ mAb/dex (100 ng/ml) and IL-4 for 4 d. (b) CSR to IgG1 in B cells stimulated with different concentrations of Pam3CSK4 and LPS in the presence of anti–δ mAb/dex (100 ng/ml) and IL-4 for 4 d.

Article Snippet: Applications Mouse B220, PE-conjugated Rat mAb eBioscience 25-0452-83 FCM Mouse B220, APC-conjugated Rat mAb BD Pharmingen 553092 FCM Mouse CD138, biotin-conjugated Rat mAb BD Pharmingen 553713 FCM Mouse Ig Goat pAb Southern Biotech 1012-01 ELISA Mouse IgG Goat pAb Southern Biotech 1030-01 ELISA Mouse IgM, FITC-conjugated Rat mAb BD Pharmingen 553437 FCM Mouse IgG1, FITC-conjugated Rat mAb BD Pharmingen 553443 FCM Mouse IgG1, APC-conjugated Rat mAb BD Pharmingen 550874 FCM Mouse IgG1, biotin-conjugated Goat pAb Southern Biotech 1070-08 ELISA Mouse IgG2a, FITC-conjugated Rat mAb BD Pharmingen 553390 FCM Mouse IgG2b, biotin-conjugated Goat pAb Southern Biotech 1090-08 FCM Mouse IgG3, FITC-conjugated Rat mAb BD Biosciences 553403 FCM Mouse IgG3, biotin-conjugated Goat pAb Southern Biotech 1100-08 ELISA Mouse IgA, FITC-conjugated Rat mAb BD Pharmingen 559354 FCM Mouse IgA, biotin-conjugated Goat pAb Southern Biotech 1040-08 FCM, ELISA Mouse IgE, PE-conjugated Rat mAb eBioscience Dec-92 FCM Open in a separate window Abbreviations: mAb, monoclonal antibody; pAb, polyclonal antibody; FCM, flow cytometry; ELISA, enzyme-linked immunosorbent assay.

Techniques: Flow Cytometry, Labeling

Journal: Autoimmunity

Article Title: B cell TLR1/2, TLR4, TLR7 and TLR9 interact in induction of class switch DNA recombination: modulation by BCR and CD40, and relevance to T-independent antibody responses

doi: 10.3109/08916934.2014.993027

Figure Lengend Snippet: LPS-induced CSR is inhibited by CpG and R-848 in the absence of BCR crosslinking (flow cytometry analysis). (a) The proportion of switched IgG1+IgM− B cells and IgM+IgG1− unswitched B cells in B cells after stimulation with LPS (3 μg/ml) plus IL-4 in the presence of nil, Pam3CSK4, R-848, CpG or GpC for 4 d. (b) CSR to IgG1 in CFSE-labeled B cells stimulated with LPS (3 μg/ml) plus IL-4 in the absence or presence of GpC (0.3 μM) or CpG (0.3 μM; left panels) for 4 d and depiction of the proportion of switched IgG1+ cells in B cells that had completed each cell division (right panels). (c) CSR to different IgG isotypes or IgA in B cells stimulated with LPS (3 μg/ml) plus appropriate cytokines (as indicated) in the absence or presence of CpG (0.3 μM) (mean and s.d. of data from three independent experiments; p values calculated by paired student t test). Data in (a) and (b) are representative of three independent experiments.

Article Snippet: Applications Mouse B220, PE-conjugated Rat mAb eBioscience 25-0452-83 FCM Mouse B220, APC-conjugated Rat mAb BD Pharmingen 553092 FCM Mouse CD138, biotin-conjugated Rat mAb BD Pharmingen 553713 FCM Mouse Ig Goat pAb Southern Biotech 1012-01 ELISA Mouse IgG Goat pAb Southern Biotech 1030-01 ELISA Mouse IgM, FITC-conjugated Rat mAb BD Pharmingen 553437 FCM Mouse IgG1, FITC-conjugated Rat mAb BD Pharmingen 553443 FCM Mouse IgG1, APC-conjugated Rat mAb BD Pharmingen 550874 FCM Mouse IgG1, biotin-conjugated Goat pAb Southern Biotech 1070-08 ELISA Mouse IgG2a, FITC-conjugated Rat mAb BD Pharmingen 553390 FCM Mouse IgG2b, biotin-conjugated Goat pAb Southern Biotech 1090-08 FCM Mouse IgG3, FITC-conjugated Rat mAb BD Biosciences 553403 FCM Mouse IgG3, biotin-conjugated Goat pAb Southern Biotech 1100-08 ELISA Mouse IgA, FITC-conjugated Rat mAb BD Pharmingen 559354 FCM Mouse IgA, biotin-conjugated Goat pAb Southern Biotech 1040-08 FCM, ELISA Mouse IgE, PE-conjugated Rat mAb eBioscience Dec-92 FCM Open in a separate window Abbreviations: mAb, monoclonal antibody; pAb, polyclonal antibody; FCM, flow cytometry; ELISA, enzyme-linked immunosorbent assay.

Techniques: Flow Cytometry, Labeling

Journal: Autoimmunity

Article Title: B cell TLR1/2, TLR4, TLR7 and TLR9 interact in induction of class switch DNA recombination: modulation by BCR and CD40, and relevance to T-independent antibody responses

doi: 10.3109/08916934.2014.993027

Figure Lengend Snippet: LPS-induced plasma cell differentiation and secretion of class-switched Ig are inhibited by CpG in the absence of BCR crosslinking. (a) Formation of B220loCD138hi plasma cells in cultures of B cells stimulated with LPS (1 μg/ml) plus IL-4 in the absence or presence of CpG (0.3 μM) for 4 d (flow cytometry analysis). (b) Formation of B220loCD138hi plasma cells in cultures of B cells stimulated with LPS plus IL-4, nil or IL-4, IL-5 and TGF-β, as indicated, in the absence or presence of CpG for 4 d (flow cytometry analysis). (c) ELISA of IgG1, IgG3 or IgA secreted into the supernatant of B cell cultures after stimulation with LPS plus IL-4, nil or IL-4, IL-5 and TGF-β, as indicated, in the absence or presence of CpG for 4 d. Data are mean and s.d. from three independent experiments (p values calculated by paired student t test).

Article Snippet: Applications Mouse B220, PE-conjugated Rat mAb eBioscience 25-0452-83 FCM Mouse B220, APC-conjugated Rat mAb BD Pharmingen 553092 FCM Mouse CD138, biotin-conjugated Rat mAb BD Pharmingen 553713 FCM Mouse Ig Goat pAb Southern Biotech 1012-01 ELISA Mouse IgG Goat pAb Southern Biotech 1030-01 ELISA Mouse IgM, FITC-conjugated Rat mAb BD Pharmingen 553437 FCM Mouse IgG1, FITC-conjugated Rat mAb BD Pharmingen 553443 FCM Mouse IgG1, APC-conjugated Rat mAb BD Pharmingen 550874 FCM Mouse IgG1, biotin-conjugated Goat pAb Southern Biotech 1070-08 ELISA Mouse IgG2a, FITC-conjugated Rat mAb BD Pharmingen 553390 FCM Mouse IgG2b, biotin-conjugated Goat pAb Southern Biotech 1090-08 FCM Mouse IgG3, FITC-conjugated Rat mAb BD Biosciences 553403 FCM Mouse IgG3, biotin-conjugated Goat pAb Southern Biotech 1100-08 ELISA Mouse IgA, FITC-conjugated Rat mAb BD Pharmingen 559354 FCM Mouse IgA, biotin-conjugated Goat pAb Southern Biotech 1040-08 FCM, ELISA Mouse IgE, PE-conjugated Rat mAb eBioscience Dec-92 FCM Open in a separate window Abbreviations: mAb, monoclonal antibody; pAb, polyclonal antibody; FCM, flow cytometry; ELISA, enzyme-linked immunosorbent assay.

Techniques: Cell Differentiation, Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: Autoimmunity

Article Title: B cell TLR1/2, TLR4, TLR7 and TLR9 interact in induction of class switch DNA recombination: modulation by BCR and CD40, and relevance to T-independent antibody responses

doi: 10.3109/08916934.2014.993027

Figure Lengend Snippet: CD154-induced B cell class switching and differentiation into PNAhi B cells and plasma cells are inhibited by CpG in the absence of BCR crosslinking (flow cytometry analysis). (a) CSR to IgG1 (top panels) and expression of PNA-binding lectins (bottom panels) in B cells stimulated with CD154 (3 unit/ml) plus IL-4 in the absence or presence of CpG (0.3 μM) for 4 d. (b) CSR to IgG1 (top panels) and expression of PNA-binding lectins (bottom panels) in B cells stimulated with CD154 (3 unit/ml) plus IFN-γ in the absence or presence of CpG for 4 d. (c) CSR to IgG1, IgE or IgG2a in B cells stimulated with CD154 plus IL-4 or IFN-γ in the absence or presence of CpG for 4 d. Data are mean and s.d. from three independent experiments (p values calculated by paired student t test). (d) Formation of B220loCD138hi plasma cells in cultures of B cells stimulated with LPS (1 μg/ml) plus IL-4 or IFN-γ in the absence or presence of CpG (0.3 μM) for 4 d. Data are mean and s.d. from three independent experiments (p values calculated by paired student t test).

Article Snippet: Applications Mouse B220, PE-conjugated Rat mAb eBioscience 25-0452-83 FCM Mouse B220, APC-conjugated Rat mAb BD Pharmingen 553092 FCM Mouse CD138, biotin-conjugated Rat mAb BD Pharmingen 553713 FCM Mouse Ig Goat pAb Southern Biotech 1012-01 ELISA Mouse IgG Goat pAb Southern Biotech 1030-01 ELISA Mouse IgM, FITC-conjugated Rat mAb BD Pharmingen 553437 FCM Mouse IgG1, FITC-conjugated Rat mAb BD Pharmingen 553443 FCM Mouse IgG1, APC-conjugated Rat mAb BD Pharmingen 550874 FCM Mouse IgG1, biotin-conjugated Goat pAb Southern Biotech 1070-08 ELISA Mouse IgG2a, FITC-conjugated Rat mAb BD Pharmingen 553390 FCM Mouse IgG2b, biotin-conjugated Goat pAb Southern Biotech 1090-08 FCM Mouse IgG3, FITC-conjugated Rat mAb BD Biosciences 553403 FCM Mouse IgG3, biotin-conjugated Goat pAb Southern Biotech 1100-08 ELISA Mouse IgA, FITC-conjugated Rat mAb BD Pharmingen 559354 FCM Mouse IgA, biotin-conjugated Goat pAb Southern Biotech 1040-08 FCM, ELISA Mouse IgE, PE-conjugated Rat mAb eBioscience Dec-92 FCM Open in a separate window Abbreviations: mAb, monoclonal antibody; pAb, polyclonal antibody; FCM, flow cytometry; ELISA, enzyme-linked immunosorbent assay.

Techniques: Flow Cytometry, Expressing, Binding Assay

Journal: Autoimmunity

Article Title: B cell TLR1/2, TLR4, TLR7 and TLR9 interact in induction of class switch DNA recombination: modulation by BCR and CD40, and relevance to T-independent antibody responses

doi: 10.3109/08916934.2014.993027

Figure Lengend Snippet: CpG-mediated inhibition of LPS- or CD154-induced CSR requires co-stimulation of B cells by CpG with LPS or CD154. (a) Levels of transcripts, as indicated, in B cells stimulated with CpG plus IL-4 for 0, 4 or 24 h (real-time qRT-PCR analysis). (b, c) CSR to IgG1 in CFSE-labeled B cells stimulated with LPS (3 μg/ml, b) or CD154 (3 unit/ml, c) plus CpG (0.3 mM) for the period as indicated plus IL4 (flow cytometry analysis). Data are representative of three independent experiments.

Article Snippet: Applications Mouse B220, PE-conjugated Rat mAb eBioscience 25-0452-83 FCM Mouse B220, APC-conjugated Rat mAb BD Pharmingen 553092 FCM Mouse CD138, biotin-conjugated Rat mAb BD Pharmingen 553713 FCM Mouse Ig Goat pAb Southern Biotech 1012-01 ELISA Mouse IgG Goat pAb Southern Biotech 1030-01 ELISA Mouse IgM, FITC-conjugated Rat mAb BD Pharmingen 553437 FCM Mouse IgG1, FITC-conjugated Rat mAb BD Pharmingen 553443 FCM Mouse IgG1, APC-conjugated Rat mAb BD Pharmingen 550874 FCM Mouse IgG1, biotin-conjugated Goat pAb Southern Biotech 1070-08 ELISA Mouse IgG2a, FITC-conjugated Rat mAb BD Pharmingen 553390 FCM Mouse IgG2b, biotin-conjugated Goat pAb Southern Biotech 1090-08 FCM Mouse IgG3, FITC-conjugated Rat mAb BD Biosciences 553403 FCM Mouse IgG3, biotin-conjugated Goat pAb Southern Biotech 1100-08 ELISA Mouse IgA, FITC-conjugated Rat mAb BD Pharmingen 559354 FCM Mouse IgA, biotin-conjugated Goat pAb Southern Biotech 1040-08 FCM, ELISA Mouse IgE, PE-conjugated Rat mAb eBioscience Dec-92 FCM Open in a separate window Abbreviations: mAb, monoclonal antibody; pAb, polyclonal antibody; FCM, flow cytometry; ELISA, enzyme-linked immunosorbent assay.

Techniques: Inhibition, Quantitative RT-PCR, Labeling, Flow Cytometry

Journal: Autoimmunity

Article Title: B cell TLR1/2, TLR4, TLR7 and TLR9 interact in induction of class switch DNA recombination: modulation by BCR and CD40, and relevance to T-independent antibody responses

doi: 10.3109/08916934.2014.993027

Figure Lengend Snippet: Co-stimulation of B cells by LPS and CD154 hampers CSR and plasma cell differentiation (flow cytometry analysis). (a, b) CSR to IgG1 (a) and formation of B220loCD138hi plasma cells (b) in B cell cultures after stimulation with LPS (3 μg/ml), CD154 (3 unit/ml) or both plus IL-4 for 4 d. Data are representative of three independent experiments.

Article Snippet: Applications Mouse B220, PE-conjugated Rat mAb eBioscience 25-0452-83 FCM Mouse B220, APC-conjugated Rat mAb BD Pharmingen 553092 FCM Mouse CD138, biotin-conjugated Rat mAb BD Pharmingen 553713 FCM Mouse Ig Goat pAb Southern Biotech 1012-01 ELISA Mouse IgG Goat pAb Southern Biotech 1030-01 ELISA Mouse IgM, FITC-conjugated Rat mAb BD Pharmingen 553437 FCM Mouse IgG1, FITC-conjugated Rat mAb BD Pharmingen 553443 FCM Mouse IgG1, APC-conjugated Rat mAb BD Pharmingen 550874 FCM Mouse IgG1, biotin-conjugated Goat pAb Southern Biotech 1070-08 ELISA Mouse IgG2a, FITC-conjugated Rat mAb BD Pharmingen 553390 FCM Mouse IgG2b, biotin-conjugated Goat pAb Southern Biotech 1090-08 FCM Mouse IgG3, FITC-conjugated Rat mAb BD Biosciences 553403 FCM Mouse IgG3, biotin-conjugated Goat pAb Southern Biotech 1100-08 ELISA Mouse IgA, FITC-conjugated Rat mAb BD Pharmingen 559354 FCM Mouse IgA, biotin-conjugated Goat pAb Southern Biotech 1040-08 FCM, ELISA Mouse IgE, PE-conjugated Rat mAb eBioscience Dec-92 FCM Open in a separate window Abbreviations: mAb, monoclonal antibody; pAb, polyclonal antibody; FCM, flow cytometry; ELISA, enzyme-linked immunosorbent assay.

Techniques: Cell Differentiation, Flow Cytometry